exosome drug loading
The exploitation of exosomes as drug delivery vehicles offers important advantages compared to other nanoparticulate drug delivery systems such as liposomes and polymeric nanoparticles; exosomes are non-immunogenic in nature due to similar composition as body׳s own cells.
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Dec 10, 2015 · The drug loading was determined by HPLC and varied from 7.2% for PTX to 11.7% for Dox. It is worth noting that exosomes are a very distinct type of nanocarrier that, by their nature, already carries numerous proteins and nucleic acids. This may explain the relatively low loading capacity achieved with these carriers.
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Exosomes are cell-derived vesicles that are present in many and perhaps all biological fluids, including blood, urine, and cultured medium of cell cultures.
Mar 08, 2016 · Exosomes as therapeutic drug carriers and delivery vehicles across biological membranes: current perspectives and future challenges There exist many challenges in a clear understanding of exosomes with regard to therapeutic cargo loading and assembly for drug delivery. Exosomes as drug delivery vehicles offer a major
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However, the de- velopment of exosomes as drug delivery vehicles will require drug loading strategies that are different from those used in loading lipo- somes. In principle, exosomes could be loaded either in vivo during their biogenesis or in vitro in puriﬁed exosomes (Fig. 2).
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The blood–brain barrier (BBB) essentially restricts therapeutic drugs from entering into the brain. This study tests the hypothesis that brain endothelial cell derived exosomes can deliver anticancer drug across the BBB for the treatment of brain cancer in a zebrafish (Danio rerio) model.
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Exosomes are simply incubated with drugs, and the drugs diffuse into the exosomes along the concentration gradient. The loading efficiency depends on the hydrophobicity of the drug molecules.
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Oct 16, 2018 · A drug-exosome mixture was sonicated for six cycles of 30 s on/off for a total of 3 min with 2 min cooling period, which resulted in effective drug loading into exosomes. Taking the size, the zeta potential and the quantity of drug loading into account, there are no significant changes in the structure and content of exosomal membranes after sonication .
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(d) Loading efficiency of various protein-loaded exosomes was calculated by dividing the number of luciferase molecules in exosomes with the number of luciferase molecules in the exosome-producing
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Exosomes derived from mesenchymal stem cells (MSCs) have been evaluated for their potential to be used as drug delivery vehicles. Synthetically personalized exosome mimetics (EMs) could be the alternative vesicles for drug delivery. In this study, we aimed to isolate EMs from human MSCs.
Author: Senthilkumar Kalimuthu, Senthilkumar Kalimuthu, Prakash Gangadaran, Prakash Gangadaran, Ramya Lakshm
In vitro drug loading. In vitro drug loading refers to the loading of drugs into purified preparations of exosome. Since exosomes resemble liposomes in having a bilipid membrane and an aqueous core, they could potentially be loaded with both hydrophilic and lipophilic drugs.
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Putting exosomes to work: Load your cargo directly into isolated exosomes With Exo-Fect, you can turn isolated exosomes into cargo delivery vehicles that introduce RNAs, DNAs (including plasmids), and even small molecules into recipient cells.
Aug 17, 2018 · In this video for Evox Therapeutics we explore how exosomes – the body’s own delivery system – can be used to deliver drugs in a precise, targeted way.
loading method for the efficient encapsulation of anti-inflammatory drugs will be developed and the efficacy of drug-loaded exosomes will be tested in relevant in vitro and in vivo models.